Increase of enzyme activity through specific covalent modification with fragments.

Increase of enzyme activity through specific covalent modification with fragments† †Electronic supplementary information (ESI) available: Description of the experimental methods, chemical synthesis and analysis, details of the results and supplementary figures and tables. See DOI: 10.1039/c7sc01966a Click here for additional data file.

Irreversible inhibitors of the 3C protease of Coxsackie virus through templated assembly of protein-binding fragments

Small-molecule fragments binding to biomacromolecules can be starting points for the development of drugs, but are often difficult to detect due to low affinities. Here we present a strategy that identifies protein-binding fragments through their potential to induce the target-guided formation of covalently bound, irreversible enzyme inhibitors. A protein-binding nucleophile reacts reversibly w...

کاربرد نانوذرات مغناطیسی آهن اکسید در فرآیند تثبیت بیومولکول‌های زیستی

Introduction: Because of their unique properties, magnetic nanoparticles have attracted the attention of many researchers in various fields. The stabilization enzyme on functionalized magnetic nanoparticles, with the maintenance of free protein activity and optimal stability, have been developed by various surface modification techniques. This review focused on the methods for  modificatio...

Influence of a Novel Magnetic Recoverable Support on Kinetic, Stability and Activity of Beta-amylase Enzyme

In this paper, covalent immobilization of beta amylase enzyme on the surface of modified magnetic nano particles (ZnFe2O4@SiO2-NH2) is reported. For doing so, at first, the magnetic nanoparticles of ZnFe2O4 were synthesized by chemical co-precipitation method and then tetraethyl orthosilicate (TEOS) and 3-aminopropyltriethoxy sil...

Mechanisms of hormone action.

Circulating hormones play an important role in regulating the flux through metabolic pathways. In general, hormones regulate either the activity of key enzymes of metabolism or the activity of protein transport systems such as those found in the plasma membrane and in the inner mitochondrial membrane. Little is known about the mechanism of action of hormones on transport systems, so the discuss...